Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures.
Identifieur interne : 000D65 ( Main/Exploration ); précédent : 000D64; suivant : 000D66Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures.
Auteurs : S T Ten [Malaisie] ; U. Hashim [Malaisie] ; S C B. Gopinath [Malaisie] ; W W Liu [Malaisie] ; K L Foo [Malaisie] ; S T Sam [Malaisie] ; S F A. Rahman [Malaisie] ; C H Voon [Malaisie] ; A N Nordin [Malaisie]Source :
- Biosensors & bioelectronics [ 1873-4235 ] ; 2017.
Descripteurs français
- KwdFr :
- ADN bactérien (), ADN bactérien (isolement et purification), ADN simple brin (), ADN simple brin (isolement et purification), Escherichia coli O157 (isolement et purification), Escherichia coli O157 (pathogénicité), Humains, Microbiologie alimentaire, Nanostructures (), Or (), Silice (), Son (acoustique), Techniques de biocapteur ().
- MESH :
- isolement et purification : ADN bactérien, ADN simple brin, Escherichia coli O157.
- pathogénicité : Escherichia coli O157.
- ADN bactérien, ADN simple brin, Humains, Microbiologie alimentaire, Nanostructures, Or, Silice, Son (acoustique), Techniques de biocapteur.
English descriptors
- KwdEn :
- Biosensing Techniques (methods), DNA, Bacterial (chemistry), DNA, Bacterial (isolation & purification), DNA, Single-Stranded (chemistry), DNA, Single-Stranded (isolation & purification), Escherichia coli O157 (isolation & purification), Escherichia coli O157 (pathogenicity), Food Microbiology, Gold (chemistry), Humans, Nanostructures (chemistry), Silicon Dioxide (chemistry), Sound.
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Single-Stranded, Gold, Silicon Dioxide.
- chemical , isolation & purification : DNA, Bacterial, DNA, Single-Stranded.
- chemistry : Nanostructures.
- isolation & purification : Escherichia coli O157.
- methods : Biosensing Techniques.
- pathogenicity : Escherichia coli O157.
- Food Microbiology, Humans, Sound.
Abstract
Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1cfu E.coli O157:H7 in 25g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130.5nm thin layer of SiO2 nanostructures with particle size lesser than 70nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide target and attained the sensitivity of 0.6439nM/0.1kHz and detection limit was down to 1.8femto-molar (1.8×10-15M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.
DOI: 10.1016/j.bios.2016.09.035
PubMed: 27660016
Affiliations:
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Hashim</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, Arau 02600, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, Arau 02600</wicri:regionArea><wicri:noRegion>Arau 02600</wicri:noRegion></affiliation></author><author><name sortKey="Gopinath, S C B" sort="Gopinath, S C B" uniqKey="Gopinath S" first="S C B" last="Gopinath">S C B. Gopinath</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400</wicri:regionArea><wicri:noRegion>Serdang 43400</wicri:noRegion></affiliation></author><author><name sortKey="Liu, W W" sort="Liu, W W" uniqKey="Liu W" first="W W" last="Liu">W W Liu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000</wicri:regionArea><wicri:noRegion>Kangar 01000</wicri:noRegion></affiliation></author><author><name sortKey="Foo, K L" sort="Foo, K L" uniqKey="Foo K" first="K L" last="Foo">K L Foo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000</wicri:regionArea><wicri:noRegion>Kangar 01000</wicri:noRegion></affiliation></author><author><name sortKey="Sam, S T" sort="Sam, S T" uniqKey="Sam S" first="S T" last="Sam">S T Sam</name><affiliation wicri:level="1"><nlm:affiliation>School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau 02600, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau 02600</wicri:regionArea><wicri:noRegion>Arau 02600</wicri:noRegion></affiliation></author><author><name sortKey="Rahman, S F A" sort="Rahman, S F A" uniqKey="Rahman S" first="S F A" last="Rahman">S F A. Rahman</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400</wicri:regionArea><wicri:noRegion>Serdang 43400</wicri:noRegion></affiliation></author><author><name sortKey="Voon, C H" sort="Voon, C H" uniqKey="Voon C" first="C H" last="Voon">C H Voon</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000</wicri:regionArea><wicri:noRegion>Kangar 01000</wicri:noRegion></affiliation></author><author><name sortKey="Nordin, A N" sort="Nordin, A N" uniqKey="Nordin A" first="A N" last="Nordin">A N Nordin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Electrical and Computer Engineering, Engineering Faculty, International Islamic University Malaysia, Kuala Lumpur 53100, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Department of Electrical and Computer Engineering, Engineering Faculty, International Islamic University Malaysia, Kuala Lumpur 53100</wicri:regionArea><wicri:noRegion>Kuala Lumpur 53100</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biosensors & bioelectronics</title><idno type="eISSN">1873-4235</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (isolation & purification)</term><term>DNA, Single-Stranded (chemistry)</term><term>DNA, Single-Stranded (isolation & purification)</term><term>Escherichia coli O157 (isolation & purification)</term><term>Escherichia coli O157 (pathogenicity)</term><term>Food Microbiology</term><term>Gold (chemistry)</term><term>Humans</term><term>Nanostructures (chemistry)</term><term>Silicon Dioxide (chemistry)</term><term>Sound</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien ()</term><term>ADN bactérien (isolement et purification)</term><term>ADN simple brin ()</term><term>ADN simple brin (isolement et purification)</term><term>Escherichia coli O157 (isolement et purification)</term><term>Escherichia coli O157 (pathogénicité)</term><term>Humains</term><term>Microbiologie alimentaire</term><term>Nanostructures ()</term><term>Or ()</term><term>Silice ()</term><term>Son (acoustique)</term><term>Techniques de biocapteur ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Single-Stranded</term><term>Gold</term><term>Silicon Dioxide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Single-Stranded</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanostructures</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Escherichia coli O157</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN bactérien</term><term>ADN simple brin</term><term>Escherichia coli O157</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Escherichia coli O157</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Escherichia coli O157</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Food Microbiology</term><term>Humans</term><term>Sound</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN bactérien</term><term>ADN simple brin</term><term>Humains</term><term>Microbiologie alimentaire</term><term>Nanostructures</term><term>Or</term><term>Silice</term><term>Son (acoustique)</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1cfu E.coli O157:H7 in 25g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO<sub>3</sub> substrate. Its sensitivity was enhanced by depositing 130.5nm thin layer of SiO<sub>2</sub> nanostructures with particle size lesser than 70nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH<sub>2</sub>)<sub>3</sub>-CHO) and APTES; NH<sub>2</sub>-(CH<sub>2</sub>)<sub>3</sub>-Si(OC<sub>2</sub>H<sub>5</sub>)<sub>3</sub>]. The high-performance of sensor was shown with the specific oligonucleotide target and attained the sensitivity of 0.6439nM/0.1kHz and detection limit was down to 1.8femto-molar (1.8×10<sup>-15</sup>M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.</div></front></TEI><affiliations><list><country><li>Malaisie</li></country></list><tree><country name="Malaisie"><noRegion><name sortKey="Ten, S T" sort="Ten, S T" uniqKey="Ten S" first="S T" last="Ten">S T Ten</name></noRegion><name sortKey="Foo, K L" sort="Foo, K L" uniqKey="Foo K" first="K L" last="Foo">K L Foo</name><name sortKey="Gopinath, S C B" sort="Gopinath, S C B" uniqKey="Gopinath S" first="S C B" last="Gopinath">S C B. Gopinath</name><name sortKey="Hashim, U" sort="Hashim, U" uniqKey="Hashim U" first="U" last="Hashim">U. Hashim</name><name sortKey="Liu, W W" sort="Liu, W W" uniqKey="Liu W" first="W W" last="Liu">W W Liu</name><name sortKey="Nordin, A N" sort="Nordin, A N" uniqKey="Nordin A" first="A N" last="Nordin">A N Nordin</name><name sortKey="Rahman, S F A" sort="Rahman, S F A" uniqKey="Rahman S" first="S F A" last="Rahman">S F A. Rahman</name><name sortKey="Sam, S T" sort="Sam, S T" uniqKey="Sam S" first="S T" last="Sam">S T Sam</name><name sortKey="Voon, C H" sort="Voon, C H" uniqKey="Voon C" first="C H" last="Voon">C H Voon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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